Exhibit 10.16

THIRD AMENDMENT TO LICENSE AGREEMENT

Agreement # LIC18110

This Third Amendment is made and entered into on the 8^th day of June, 2023 (“Effective Date”), by and between the UNIVERSITY OF SOUTH FLORIDA RESEARCH FOUNDATION, INC. (hereinafter referred to as “Licensor”), a corporation not for profit under Chapter 617 Florida Statutes, and a direct support organization of the University of South Florida pursuant to section 1004.28 Florida Statutes, and Alzamend Neuro, Inc., classified as a corporation organized and existing under the laws of Delaware (hereinafter referred to as “Licensee”).

WHEREAS, on July 2, 2018, Licensor and Licensee entered into a license agreement relating to the utilization of Patent Rights (“License Agreement”) associated with USF Technology referenced as 12B100_Shytle;

WHEREAS, a Second Amendment to the License Agreement was made effective March 30, 2021; and

WHEREAS, the parties desire to further amend the License Agreement in this Third Amendment to the License Agreement.

NOW, THEREFORE, the parties agree as follows:

Capitalized terms in this Third Amendment shall have the same meaning as set forth in the License Agreement, unless defined otherwise in this Third Amendment. All other terms and conditions of the License Agreement shall continue in full force and effect. This Third Amendment, together with the License Agreement constitutes the entire agreement between the parties hereto regarding the subject matter hereof and supersedes any prior and/or contemporaneous agreement(s), understanding(s) and/or negotiation(s).

1.13 “First Commercial Sale” means the first commercial sale, lease or other transfer, practice or disposition of any Licensed Product or Licensed Process for value, in any country by Licensee or by a Sublicensee to a third party that is not a Licensee Affiliate or a Sublicensee.

Licensee will pay to Licensor minimum annual royalty payments beginning in the calendar year in which the First Commercial Sale occurs as follows:

The minimum royalty shall be paid in advance on a quarterly basis for each year in which this Agreement is in effect. The minimum royalty for a given year shall be due in advance and shall be paid in quarterly installments on March 31, June 30, September 30, and December 31 for the following quarter. Any minimum royalty paid in a calendar year will be credited against the earned royalties for that calendar year. It is understood that the minimum royalties will be applied to earned royalties on a calendar year basis, and that sales of Licensed Products and/or Licensed Processes requiring the payment of earned royalties made during a prior or subsequent calendar year shall have no effect on the annual minimum royalty due Licensor for other than the same calendar year in which the royalties were earned.

IN WITNESS WHEREOF, the parties have set their hands and seals and duly executed this Amendment as of the Effective Date identified in the preamble above.

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